27th Congress of the French Association of Cytometry in Marseille

Chèr(e)s ami(e)s et collègues,

Friends and colleagues,

It is with great pleasure that we invite you to the 26th the French Association of Cytometry (AFC) from december 17 to 19, 2024 which will take place in Marseille. Program and registration on the dedicated website.

FSEV-AFC Hands-on Workshop 2024

Dear members

The first HANDS-ON WORKSHOP: “Single Extracellular Vesicles analysis” is jointly organized by the French Society of Extracellular Vesicles (FSEV)and the French Association of Cytometry (AFC) and will take place from February 7 to 9, 2024 at FIAP Jean Monnet,, in Paris

Extracellular vesicles are gaining increasing attention from researchers and clinicians due to their pivotal roles in various physiological and pathological processes. They influence the progression and treatment responses of several diseases, including cancers. Additionally, extracellular vesicles hold promise as disease biomarkers, offering non-invasive diagnostic potential. The analysis of single extracellular vesicles is now emerging as the main challenge in the field of extracellular vesicles.

This workshop will cover the basic and advanced technology dedicated to single extracellular vesicles analysis and will consist of lectures by international experts in the field and hands-on pratical modules animated by the following companies: Abbelight, Agilent, BD, Beckman Coulter, Cytek Biosciences, Excilone & Particle Metrix, IZON, Miltenyi, Myriade and NanoFCM.

The workshop will be organized as follow over 3 days:

  • An afternoon dedicated to an overview of the fundamental and technological challenges in the study of extracellular vesicles by academic experts.
  • A full day with practical sessions divided into 4 slots of 2 hours where each company will allow groups of 6 people to manipulate directly their device and to test the reference material of the company and batches of calibrated vesicles.
  • A Morning dedicated to the analysis and the discussion of the results on each technology.

Detailed desciptions of modules and faculty bios will be available on this site.

Please note, places are limited, register quickly!!!

With best regards, on behalf of the Organizing Committee

Mass Cytometry group AFC : Characterization of Regulatory T Lymphocytes in Breast Cancers

The Mass Cytometry Group of the French Cytometry Association Invites you to its Webinar

Tuesday November 7, 2023 at 12:30 p.m

Characterization of Regulatory T Lymphocytes in Breast Cancer

Presented by Stephane Fattori

Immunomonitoring department, Pauli Calmettes Institute, CRM, Marseille

Zoom link : https://univ amu fr.zoom.us/j/86931336119?pwd= TEgzb01ZM0FucFhKb3hEdmk5VEpKZz09
Login code: 725552

Abstract :
Triple negative breast cancer (TNBC) is the deadliest subtype of breast cancer Standard treatments for
patients with early stage, locally advanced or metastatic TNBC include the combination of neoadjuvant
chemotherapy and pembrolizumab, an immune checkpoint blockade targeting the programmed cell
death 1 (PD-1) receptor PD-1 blockade reinvigorate tumor antigen specific CD8+ T cells in primary TNBC.
However, approximately 40 and 80 of early stage and advanced or metastatic TNBC are resistant to
PD 1 blockade therapy, respectively.
Regulatory T cells (Tregs) are an immunosuppressive subset of CD4+ T cells essential for maintaining self
tolerance in physiological settings. Abundant tumor infiltration by Tregs harbouring hallmarks of highly
suppressive CD25high effector Tregs (eTregs) has been associated with resistance to PD 1 blockade in
cancer indications for which pembrolizumab was approved much earlier than for TNBC, such as non
small cell lung cancer. In contrast, in TNBC, the prognostic value of intratumoral Tregs remains
controversial and no association between baseline levels of eTregs and responses to PD 1 blockade
therapy has been reported, despite the clinical significance of this cancer indication. In the present
thesis, we used single cell technologies to dissect the nature and function of eTreg during TNBC
progression. We found that an immunosuppressive TNBC microenvironment resistant to PD-1 blockade
therapy is marked by an imbalance between effector CD8+ T cells and CD25 high eTregs. We further
identified CD25 as the most selective surface marker of eTregs in primary TNBC and associated
metastases for limited on target/off target effects. In a syngeneic TNBC model, selective Treg depletion
by a next generation anti-CD25 monoclonal antibody (mAb; ALD 2510; Alderaan Biotechnology)
synergized with PD-1 blockade to safely mediate durable tumor growth control by increasing effector
αβCD8+ T cell/Treg ratios in tumors and in the peripheral blood Overall, we propose that Treg depletion
by next generation anti-CD25 mAb could improve clinical responses to PD-1 blockade therapy in patients
with TNBC at different stage of the disease

EasyPanel Webinar: Automated/AI-guided Flow Cytometry Panel Designer

At the Cyto conference in Montreal 2 weeks ago, we picked up (flow-cytometry.net), a tool that uses AI to instantly designate optimized panels of cytometry (conventional and spectral). We therefore wish to communicate this information to the French cytometry community.

EasyPanel is a French startup that has developed an algorithm that automatically offers panels that minimize spillover/complexity/similarity score, match antigens to fluorochromes (based on expression/coexpression levels and fluorochrome brightness) and identify commercially available products (the tool is directly integrated with the commercial database of all suppliers, and is diagnostic of it).

About a hundred universities/pharma companies already use this tool, most of them in the US (but also a few in France: Pasteur, Servier, IGR…).

A webinar is therefore offered to you on June 29 to discover the interest of this software in your laboratories.

Follow the Webinar (Microsoft Teams) on June 29 at 11 a.m ICI 

Please feel free to forward this information to other researchers, engineers, technicians, students, clinicians who do cytometry/panel design.

See you on June 29, block the date in your calendars !


Catherine Blanc ,Présidente de l’AFC

 Omar Abouzid
EasyPanel Co-founder

Data Analysis : Build an informative abundance heatmap.

The Data Analysis Group of the French Cytometry Association

Invites you to its 3nd Webinar 2023


Build an informative abundance heatmap.

Presented by :

S. Granjeaud

Plateforme Bioinformatique

Centre de Recherche en Cancérologie de Marseille, Marseille, France

Thursday June 29, 2023 / 13h00 – 14h00

Ask your questions on the forum of AFC before the webinar
Login link ZOOM at the webinar

Please spread this announcement around you.

See you soon 


Find us on the group page

Practical Days: Unsupervised analyzes in cytometry 2023

Download poster of JP !

Practical days

Unsupervised analyzes in cytometry of July 6 and 7, 2023 Sophia-Antipolis (Computer room)

Limited number of places Pré-registration !

Hello, the French Cytometry Association (AFC) is organizing the Practical Days: Unsupervised analyzes in cytometry from Thursday July 6 to Friday July 7, 2023 in Sophia-Antipolis.

Programme des Journées Pratiques

Hosted by :

  • Aïda Meghraoui-Kheddar (AMKbiotech, Sophia-Antipolis),
  • Samuel Granjeaud (CRCM, Inserm Marseille),
  • Anne-Laure iscache (Infinity, Inserm Toulouse).

Goal :

  • Introduction to unsupervised analysis methods of multi-parametric data.
  • Presentation and use of algorithms offered by Tercen, FlowJo and Cytobank.
  • Algorithms presented: t-SNE, UMAP, FlowSOM…

Audience : Technicians, engineers, students, researchers regularly practicing cytometry.

Prerequisites :

  • Good knowledge in multiparametric cytometry
  • Knowledge of the R language is not a prerequisite
  • Mastery of good practices in cytometry.

The number of places being limited, we invite you to pre-register quickly.

Pré-registration !

Please note: final registration subject to availability of places!

Upon receipt of your pre-registration and subject to availability, we will send you a detailed confirmation email, with practical information concerning the days. For regulations and order forms, please wait for receipt of final registration confirmation.

For any additional information, you can contact the administrative secretariat:

Alpha Visa Congrès / JP AFC 07 2023 624, rue des Grèzes 34070 Montpellier – France Tél. : +33 (0)4 67 03 03 00 – Fax : +33 (0)4 67 45 57 97 jtafc@alphavisa.com

Thank you for distributing this first announcement to people likely to be interested in this event. For this, we also invite you to download poster and the program.

Looking forward to the pleasure of welcoming you to Sophia-Antipolis, we remain at your disposal for any further information and please accept the expression of our sincere greetings.

Groupe Cytométrie de masse AFC : Live-Cell Barcoding with Anti-CD45 Antibodies for Mass Cytometry

Le groupe de travail “Cytométrie de masse” de l’Association Française de Cytométrie est heureux de vous inviter à son prochain webinar :

Mercredi 7 juin à 13h

Live-Cell Barcoding with Anti-CD45 Antibodies for Mass Cytometry

Présenté par Pauline Maby

Immunomonitoring department, Pauli Calmettes Institute, Marseille

Lien zoom : https://univ-amu-fr.zoom.us/j/87588252730?pwd=ek5ZQytnSEd6Q21ZM3FvZ3Bka3lQZz09

Résumé : Le codage à barres CD45 des cellules vivantes implique la coloration d’échantillons de cellules immunitaires avec des combinaisons uniques d’anticorps anti-CD45 comme identificateurs d’échantillons. Cela permet le regroupement d’échantillons afin de les traiter comme un échantillon de cellule unique pour la coloration et l’acquisition des cellules. Il améliore le flux de travail, améliore la cohérence des données et réduit l’utilisation de réactifs.

Au cours de ce webinaire, nous discuterons de toutes les étapes d’une expérience de codage à barres CD45 sur cellules vivantes, de la conception du panel à l’analyse des données. Tout d’abord, lors du panel design, il est important de s’assurer que la coloration CD45 n’interfère pas avec la détection de marqueurs d’intérêt dans d’autres canaux. Ensuite, plusieurs ajustements doivent être apportés aux procédures de coloration et d’acquisition. Enfin, les fichiers FCS obtenus à partir d’échantillons de cellules à code-barres sont décodés pour attribuer chaque événement cellulaire à son échantillon d’origine.

En espérant vous y retrouver nombreux !

A très bientôt ! 


Nous retrouver sur la page du groupe

GT Microbiologie-Cytométrie : Vitalité cellulaire

Le Groupe Microbiologie-Cytométrie de l’Association Française de Cytométrie

Vous invite à son Webinar 2023

Vitalité Cellulaire

La vitalité cellulaire dépend de la charge énergétique des cellules. Elle se traduit par la cinétique des réactions métaboliques ou activité métabolique. Dans les industries de fermentation, les ferments doivent être viables mais aussi actifs pour coloniser rapidement le milieu en début de fermentation. On peut aussi évaluer l’effet de stress sur les cellules par la mesure de vitalité. La cytométrie en flux permet de mesurer en 30min la vitalité cellulaire, ce qui est plus simple et plus rapide que les mesures cinétiques d’activités métaboliques. Ce webinaire présentera la méthode avec quelques exemples de corrélation avec des mesures d’activités sur des levures et des bactéries.

Présenté par :

Marielle BOUIX

Responsable du groupe thématique Microbiologie-Cytométrie SFM

Mardi 30 Mai 2023 / 13h30 – 14h15

Lien de connexion au webinar : Cliquez

Merci de diffuser cette annonce autour de vous.

A très bientôt ! 

Société Française de Microbiologie

36 avenue Jean Moulin 75014 Paris


secretariat@sfm-microbiologie.org / comptabilite@sfm-microbiologie.org


Nous retrouver sur la page du groupe

Questionnaire : “Lost in Translation: Cytometry in a Multilingual Setting” workshop@CYTO-2023

Dear colleagues,
We are collecting information to assess the level of interest in educational materials in languages other than English for our “Lost in Translation: Cytometry in a Multilingual Setting” workshop at CYTO in Montreal which will take place on Sunday May 21 from 15:45 to 16:45 in room 511D. 

We created a survey which will take only 5 min to answer. The survey will be due at 5pm on May 10th.

Here is the link:

Some background and a link to CYTO 2023 Program: 

Scientific communication at the higher level worldwide takes place in English and any scientist who wishes to advance their career internationally must be fluent. There is, however, a huge population of clinical, research and industrial cytometrists who are not English fluent and who are working at institutions where the primary language is not English. Often technical staff, both in research and clinical environments, are the ones operating the instruments and generating the data but are not able to follow training courses or teaching material in English. In this workshop we would like to address the issues encountered by English speaking or bilingual faculty teaching in countries where this has been a difficulty and explore ways to improve them. The second topic is to put into place a system to identify and gather educational material, whether in English or already existing in other languages, to translate into multiple languages and to make the translated material available for free access to all who can benefit.

Could you please diffuse this information and survey to anyone or groups who might be interested? Welcome any of the associated societies or persons who might be interested in participating in this endeavor for different languages to attend the workshop. If you are not coming to CYTO you can contact Zosia directly at the emails below. 

Thanks for your time and support!

Our Best Regards,

Zosia Maciorowski, zosiamaciorowski@gmail.com
Catherine Blanc 
Maggie Wang
Mariela Bollati

Survey : “Overcoming analysis paralysis ” workshop @CYTO-2023

Dear cytometry users,

We are reaching out to you, kindly asking for 3 minutes of your precious time to fill out a survey to share with us your experience using computational tools for cytometry data analysis–whatever your level of experience with these tools, even if non-existent! 

Survey link: https://forms.gle/igs2s42efQ1mB5kSA

Your feedback is needed to help us identify the barriers and pain points preventing users from adopting computational analytical tools. This knowledge will allow us to develop solutions to overcome such obstacles.

This survey is open until May 12th and results will be presented at the CYTO conference in Montreal, during the workshop: “Overcoming analysis paralysis: How to break into the high-dimensional cytometry data analysis space” that will take place on May 22nd at 3:45PM in room 510A.

Thank you for your input!

Aïda Meghraoui

Alice Wiedeman

Anne-Laure Iscache

Anna Belkina 

Giulia Grazia

Geoffrey Kraker


Data Analysis: Decryption, to read a heatmap, you have to know how to build it.

The Data Analysis Group of the French Cytometry Association

Invites you to its 2nd Webinar 2023


To read a heatmap you have to know how to build it

Presented by :

S. Granjeaud

Plateforme Bioinformatique

Centre de Recherche en Cancérologie de Marseille, Marseille, France

wednesday may 10, 2023 / 13h00 – 14h00

Ask your questions on the forum of AFC before the webinar
Login link ZOOM at the webinar

Please spread this announcement around you.

See you soon 


Find us on the group page

Mass Cytometry : Enhanced single-cell segmentation and whole slide imaging analysis through dual-modality immunofluorescen

The Mass Cytometry Group of the French Cytometry Association Invites you to its Webinar

Enhanced single-cell segmentation and whole slide imaging analysis through dual-modality immunofluorescence and imagingmass cytometry  (IMC)

Presented by : John Lizhe Zhuang

Department of Oncology, University of Cambridge

Tuesday may 2, 2023 at 16h00

login link ZOOM

Please spread this announcement around you.

See you soon 


Find us on the group page

26th Congress of the French Association of Cytometry in Toulouse

Friends and colleagues,

It is with great pleasure that we invite you to the 26th the French Association of Cytometry (AFC) from November 15 to 17, 2022 which will take place in Toulouse. Program and registration on the dedicated website.


6th German Mass Cytometry User Forum – Berlin,

Dear friends of mass cytometry, after two years of virtual meetings we are excited to announce the

6th German Mass Cytometry User Forum

The programme features invited speakers, a tutorial, selected abstracts and highlights projects from German Mass Cytometry and Imaging Mass Cytometry sites. We are glad to bring back a poster session on Thursday evening along with Wine & Cheese, providing rich opportunity for networking. Due to limited space on-site we also offer virtual participation via Zoom.

Confirmed speakers include:

  • Rosario Astaburuaga
  • Bernd Bodenmiller, Zurich, Switzerland
  • Christian Busse, Heidelberg, Germany
  • Ermelinda Porpiglia, Aarhus, Denmark

I am looking forward to an inspiring and fruitful 6th German Mass Cytometry User Forum!
Best wishes,
Henrik Mei

Meeting venue: Deutsches Rheuma-Forschungszentrum Berlin, Charitéplatz 1, 10117 Berlin

Registration is open

Short talks and poster presentations are only possible on-site. Please be aware that we have only limited space on-site.  To cover costs, we charge EUR 100 for on-site participation.

Registration for on-site participation until 6th of January 2023

Please register as soon as possible.

Registration for virtual participation

All those who register here will receive the link to the Zoom meeting in the week of the meeting.

Abstract submission until 5th of December

For short talk and poster presentation, please submit your abstract via e-mail to: masscytometry@drfz.de.

Please note your abstract will be selected by a jury either for short talk or poster presentation.

Organisation group

Henrik Mei
Marie Burns
Axel Schulz
Ute Hoffman
Jacqueline Hirscher
Elmar Endl

Dear member,

The vote for the renewal of the CA 2022 is open (to mambers only):

The results will be communicated to the congress AFC/SFI 2022 during the AFC General Assembly to be held on Wednesday November 23 from 6:45 p.m. to 7:45 p.m.

Happy voting everyone

CYTHEM : all about CD30

Dear colleagues,

CytHem is pleased to inform you that, following the MFI30 cytometry work carried out under the aegis of the AFC, a webinar entitled “All about CD30” will take place on Friday October 21 from 1 p.m. to 2:30 p.m. (program below ).

Here is the Teams link to open with Chrome :

Click here to take part to a meeting

Hope to see many of you there !

The team ‘Cythem’

Image Cytometry : Cell Cycle in Image Cytometry

The Image Cytometry Working Group of the French Association of Cytometry offers a Webinar

Monday, October 3, 2022 from 1:30 p.m. to 2:15 p.m.

Interest of image cytometry in the evaluation of the expression and localization of the TRIM37 protein during the cell cycle in a chondrocyte line

Benjamin Brigant

Centre of Molecular Inflammation Research (CEMIR),  Department of Clinical and Molecular Medicine (IKOM),NTNU, Trondheim, Norvège

Registration is mandatory via the following form:  link

Registration deadline September 28, 2022. The meeting link will be sent to you once registration has been completed.

Hope to see many of you there!

The Image Cytometry group ’

V. Duplan-Eche, S. Dussurgey, Y. Demont, M. Dussiot, C. Guérin, J. Cosette, M. Boyer-Clavel, F. Hédin, ML.  Renoud, AL. Raveu, A. Malfait-Jobart  

contact : valerie.duplan@inserm.fr

5ème Congrès FSEV 19-21 Octobre 2022

After two years of exchanging via screens, we are impatient to be able to bring together the community of EVologists during a face-to-face congress.

For the occasion, the FSEV (French Society of Extracellular Vesicles) is joining forces with the AFC (French Cytometry Association) to organize a congress in Paris from October 19 to 21, 2022.

This congress will offer you an afternoon dedicated to the contribution of flow cytometry technology in the study of extracellular vesicles.

Two days of plenary sessions, oral presentations and posters will then follow which will give you an overview of the vitality and excellence of French research on extracellular vesicles. We hope to see many of you there!

Registration and abstract submission are open. You can submit your abstracts until September 09, 2022. Registration is open until September 30, 2022.

To register you will find all the information on the dedicated page of our site. (https://www.fsev.fr/fsev-meeting-2022.html)

The organizing committees of the FSEV and the AFC.

Practical Days : Unsupervised analyzes in cytometry 2022


BD Biosciences Days

On your calendars !

The BD Biosciences team is pleased to invite you to various events during the year 2022, book your days now:

. Research day on June 29, 2022 at the Labo Business Center in paris :

  • you will also have the opportunity to register for training sessions :
    • Optional Module 1 – Software FlowJo™ level 1
    • Module 2 – Calculation of “Stain Index” and “Total Spread Matrix” in FlowJo™ software & Flow cytometry and spectral approach
    • Optional Module 3  – Software FlowJo™ level 2

For more information and registration : click here

. Clinical day on June 29, 2022 at the Labo Business Center in paris :

Please note that in addition to this clinical day, we are offering a multicolor workshop given by our Application Specialists and organized on Friday July 1st.

If you want to know more, do not hesitate to contact our training center : bdb_france_scientific_support@bd.com and/or your sales engineer

For more information and registration : click here