{"id":3546,"date":"2022-02-09T17:27:02","date_gmt":"2022-02-09T16:27:02","guid":{"rendered":"https:\/\/afcytometrie.fr\/webinar\/webinar-afc-sfm\/"},"modified":"2022-10-28T23:46:55","modified_gmt":"2022-10-28T21:46:55","slug":"webinar-afc-sfm","status":"publish","type":"webinar","link":"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/","title":{"rendered":"Webinar AFC SFM"},"content":{"rendered":"\n

The Microbiology-Cytometry SFM-AFC group offers a series of short Webinars to complement the “Microbiology” chapter of the book “Flow Cytometry” released by Lavoisier at the end of 2020. Each webinar will include the presentation of a method, detailing the protocols, for the study of microorganisms by flow cytometry, and a time for questions from participants.<\/p>\n\n\n\n

Enumeration \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 June 15\u00a0 1:30 p.m. -2:15 p.m.<\/strong><\/p>\n\n\n\n

Flow cytometry allows rapid analysis and counting of microorganisms in a suspension. Associated with fluorescent markers of viability\/mortality, it is possible to count in less than 30min the total, viable and dead yeasts or bacteria present in a suspension. This webinar will present methods and fluorescent markers for the enumeration of microorganisms, based in particular on the ISO 19344:2015 standard for the enumeration of lactic and probiotic bacteria in dairy ferments.<\/em><\/p>\n\n\n\n

Speaker\u00a0:<\/strong><\/p>\n\n\n\n

Marielle Bouix, <\/strong>Professeur Em\u00e9rite AgroParisTech<\/p>\n\n\n\n

Lien vid\u00e9o : #1_DENOMBREMENT 15\/06\/2021<\/a><\/p>\n\n\n\n

Flow-FISH\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0June 22\u00a0 1:30 p.m. -2:15 p.m.<\/strong><\/strong><\/p>\n\n\n\n

The FISH (Fluorescent In Situ Hybridization) method coupled with flow cytometry allows the specific and rapid detection of microorganisms in a mixture. After giving the principle, advantages and limits of this method, the commercial kit (VERMICON) for detecting Brettanomyces bruxellensis in wines will be presented by Pascal Brunou (SYSMEX)<\/em><\/p>\n\n\n\n

Speaker:<\/strong><\/p>\n\n\n\n

Marielle Bouix, <\/strong>Professeur Em\u00e9rite AgroParisTech                 Pascal Brunou<\/strong>, SYSMEX<\/p>\n\n\n\n

Lien vid\u00e9o#2_FLOWFISH 22\/06\/2021<\/a><\/p>\n\n\n\n

Specific detection by antibody \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0January 14, 2022 1:30 p.m. -2:15 p.m.<\/strong><\/strong><\/p>\n\n\n\n

Labeling a microorganism with a specific fluorescent antibody allows it to be detected quickly by flow cytometry. This webinar will present the principles of the method as well as elements on the choice of antibodies (polyclonal\/monoclonal), the choice of fluorochrome and coupling (direct or sandwitch method), the possibility of simultaneous labeling with a viability marker\u2026\u2026These data will be illustrated with examples.<\/em><\/p>\n\n\n\n

Speaker\u00a0:<\/strong><\/p>\n\n\n\n

Delphine Scalbert-Sterlin, <\/strong>MCU-PH   AP-HP Sorbonne Universit\u00e9 \u2013 H\u00f4pital Piti\u00e9-Salp\u00eatri\u00e8re  <\/strong><\/p>\n\n\n\n

Vincent THOMAS,PhD   <\/strong>BIOASTER Head of the Microbiome Program                        <\/strong><\/p>\n\n\n\n

Lien vid\u00e9o #3_DETECTION SPECIFIQUE PAR ANTICORPS 14\/01\/2022<\/a><\/p>\n\n\n\n

Sorting of microorganisms\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 January 22, 2022 1:30 p.m. -2:15 p.m.<\/strong><\/strong><\/p>\n\n\n\n

This webinar will present the principle, the sorting criteria (size, fluorescence) and the specificities to be taken into account when sorting microorganisms, in particular if the objective is to put them back in culture after sorting. In the case of strict anaerobic microorganisms, additional precautions are necessary to maintain anaerobiosis throughout the sorting operation. Different examples will be presented.<\/em><\/p>\n\n\n\n

Speaker\u00a0:<\/strong><\/p>\n\n\n\n

Michael BOURGE<\/strong> Imagerie-Gif, plateforme de cytom\u00e9trie CNRS – I2BC<\/p>\n\n\n\n

Vincent THOMAS,PhD   <\/strong>BIOASTER  <\/strong>Head of the Microbiome Program<\/p>\n\n\n\n

Lien vid\u00e9o #4_TRI DES MICRO-ORGANISMES 31\/01\/2022<\/a><\/p>\n","protected":false},"template":"","class_list":["post-3546","webinar","type-webinar","status-publish","hentry"],"acf":[],"yoast_head":"\nWebinar AFC SFM - AFC<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Webinar AFC SFM - AFC\" \/>\n<meta property=\"og:description\" content=\"The Microbiology-Cytometry SFM-AFC group offers a series of short Webinars to complement the “Microbiology” chapter of the book “Flow Cytometry” released by Lavoisier at the end of 2020. Each webinar will include the presentation of a method, detailing the protocols, for the study of microorganisms by flow cytometry, and a time for questions from participants. Enumeration \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 June 15\u00a0 1:30 p.m. -2:15 p.m. Flow cytometry allows rapid analysis and counting of microorganisms in a suspension. Associated with fluorescent markers of viability\/mortality, it is possible to count in less than 30min the total, viable and dead yeasts or bacteria present in a suspension. This webinar will present methods and fluorescent markers for the enumeration of microorganisms, based in particular on the ISO 19344:2015 standard for the enumeration of lactic and probiotic bacteria in dairy ferments. Speaker\u00a0: Marielle Bouix, Professeur Em\u00e9rite AgroParisTech Lien vid\u00e9o : #1_DENOMBREMENT 15\/06\/2021 Flow-FISH\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0June 22\u00a0 1:30 p.m. -2:15 p.m. The FISH (Fluorescent In Situ Hybridization) method coupled with flow cytometry allows the specific and rapid detection of microorganisms in a mixture. After giving the principle, advantages and limits of this method, the commercial kit (VERMICON) for detecting Brettanomyces bruxellensis in wines will be presented by Pascal Brunou (SYSMEX) […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/\" \/>\n<meta property=\"og:site_name\" content=\"AFC\" \/>\n<meta property=\"article:modified_time\" content=\"2022-10-28T21:46:55+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/\",\"url\":\"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/\",\"name\":\"Webinar AFC SFM - AFC\",\"isPartOf\":{\"@id\":\"https:\/\/afcytometrie.fr\/en\/#website\"},\"datePublished\":\"2022-02-09T16:27:02+00:00\",\"dateModified\":\"2022-10-28T21:46:55+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Webinars\",\"item\":\"https:\/\/afcytometrie.fr\/en\/webinars\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Webinar AFC SFM\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/afcytometrie.fr\/en\/#website\",\"url\":\"https:\/\/afcytometrie.fr\/en\/\",\"name\":\"AFC\",\"description\":\"Un site utilisant WordPress\",\"publisher\":{\"@id\":\"https:\/\/afcytometrie.fr\/en\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/afcytometrie.fr\/en\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/afcytometrie.fr\/en\/#organization\",\"name\":\"AFC\",\"url\":\"https:\/\/afcytometrie.fr\/en\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/afcytometrie.fr\/en\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/afcytometrie.fr\/wp-content\/uploads\/2021\/05\/logo-AFC.jpg\",\"contentUrl\":\"https:\/\/afcytometrie.fr\/wp-content\/uploads\/2021\/05\/logo-AFC.jpg\",\"width\":240,\"height\":87,\"caption\":\"AFC\"},\"image\":{\"@id\":\"https:\/\/afcytometrie.fr\/en\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Webinar AFC SFM - AFC","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/","og_locale":"en_US","og_type":"article","og_title":"Webinar AFC SFM - AFC","og_description":"The Microbiology-Cytometry SFM-AFC group offers a series of short Webinars to complement the “Microbiology” chapter of the book “Flow Cytometry” released by Lavoisier at the end of 2020. Each webinar will include the presentation of a method, detailing the protocols, for the study of microorganisms by flow cytometry, and a time for questions from participants. Enumeration \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 June 15\u00a0 1:30 p.m. -2:15 p.m. Flow cytometry allows rapid analysis and counting of microorganisms in a suspension. Associated with fluorescent markers of viability\/mortality, it is possible to count in less than 30min the total, viable and dead yeasts or bacteria present in a suspension. This webinar will present methods and fluorescent markers for the enumeration of microorganisms, based in particular on the ISO 19344:2015 standard for the enumeration of lactic and probiotic bacteria in dairy ferments. Speaker\u00a0: Marielle Bouix, Professeur Em\u00e9rite AgroParisTech Lien vid\u00e9o : #1_DENOMBREMENT 15\/06\/2021 Flow-FISH\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0June 22\u00a0 1:30 p.m. -2:15 p.m. The FISH (Fluorescent In Situ Hybridization) method coupled with flow cytometry allows the specific and rapid detection of microorganisms in a mixture. After giving the principle, advantages and limits of this method, the commercial kit (VERMICON) for detecting Brettanomyces bruxellensis in wines will be presented by Pascal Brunou (SYSMEX) […]","og_url":"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/","og_site_name":"AFC","article_modified_time":"2022-10-28T21:46:55+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/","url":"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/","name":"Webinar AFC SFM - AFC","isPartOf":{"@id":"https:\/\/afcytometrie.fr\/en\/#website"},"datePublished":"2022-02-09T16:27:02+00:00","dateModified":"2022-10-28T21:46:55+00:00","breadcrumb":{"@id":"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/afcytometrie.fr\/en\/webinar\/webinar-afc-sfm\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Webinars","item":"https:\/\/afcytometrie.fr\/en\/webinars\/"},{"@type":"ListItem","position":2,"name":"Webinar AFC SFM"}]},{"@type":"WebSite","@id":"https:\/\/afcytometrie.fr\/en\/#website","url":"https:\/\/afcytometrie.fr\/en\/","name":"AFC","description":"Un site utilisant WordPress","publisher":{"@id":"https:\/\/afcytometrie.fr\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/afcytometrie.fr\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/afcytometrie.fr\/en\/#organization","name":"AFC","url":"https:\/\/afcytometrie.fr\/en\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/afcytometrie.fr\/en\/#\/schema\/logo\/image\/","url":"https:\/\/afcytometrie.fr\/wp-content\/uploads\/2021\/05\/logo-AFC.jpg","contentUrl":"https:\/\/afcytometrie.fr\/wp-content\/uploads\/2021\/05\/logo-AFC.jpg","width":240,"height":87,"caption":"AFC"},"image":{"@id":"https:\/\/afcytometrie.fr\/en\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/afcytometrie.fr\/en\/wp-json\/wp\/v2\/webinar\/3546","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/afcytometrie.fr\/en\/wp-json\/wp\/v2\/webinar"}],"about":[{"href":"https:\/\/afcytometrie.fr\/en\/wp-json\/wp\/v2\/types\/webinar"}],"wp:attachment":[{"href":"https:\/\/afcytometrie.fr\/en\/wp-json\/wp\/v2\/media?parent=3546"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}